EFFECT OF 5-BROMODEOXYURIDINE ON GROWTH, ENCYSTMENT, AND EXCYSTMENT OF ACANTHAMOEBA CASTELLANII
نویسندگان
چکیده
منابع مشابه
Effect of 5-bromodeoxyuridine on Growth, Encystment, and Excystment of Acanthamoeba Castellanii
Differentiat ion of several cell types is inhibi ted by 5-bromodeoxyuridine (BUdR) (1-3, 9, 12). Since B U d R can be incorpora ted into D NA in place of thymidine (TdR) (12), the inhibi t ion of different ia t ion may be due to the presence of B U d R in the DNA of the cell. In Acanthamoeba castellanii, DNA synthesis cont inues t h rough overt different iat ion of the t rophozoite into the d o...
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Differentiation of Acanthamoeba castellanii into dormant cysts occurs spontaneously in stationary phase cultures, or can be induced experimentally by starvation. Although no further increase in cell density occurred after induction in either case, incorporation of [H]thymidine into DNA continued at a reduced rate through the period when differentiated products (cyst wall components) were formed...
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چکیده ندارد.
15 صفحه اولChanges in transfer ribonucleic acids accompanying encystment in Acanthamoeba castellanii.
Transfer ribonucleic acid (tRNA) from exponentially growing cells (trophozoites) and from precysts of Acanthamoeba castellanii were examined by reversed-phase column (RPC-2) chromatography. This system gave excellent resolution of isoaccepting species of tRNA. The tRNAs for 12 amino acids were studied. A comparison of trophozoite and precyst tRNA elution profiles revealed no apparent difference...
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Alterations in transcription that precede and accompany encystment (E) of suspension grown A. castellanii have been investigated. Comparative studies were performed on cells undergoing spontaneous E in high density stationary phase cultures or after experimental induction of E at low cell densities by deprivation of nutrients in exponential growth. Onset of growth deceleration at high cell dens...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1974
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.61.1.233